Reporter

Part:BBa_K3957000:Design

Designed by: Angus Ball, Derek Johnson, Shamitha Aravind   Group: iGEM21_Northern_BC   (2021-09-11)


A FRET based chimeric protein that binds to vitamin D


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 897
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the chassis for our system is E. coli, the part was codon optimize the part. There was an inframe stop codon in the original part (BBa_K3515011) which had to be removed. Since BioBrick was being used for the insertion of our part, codon optimization created several restriction enzyme sites which were removed to make the part RFC[10] compatible.


Source

This part is a modified version of the part BBa_K3515011.

References